Protocol detail

Histological Staining and Immunohistochemistry

Find Similar Protocols

Frozen sections (8 μm thick) were stained by using the NoVo Link Polymer Detection System (Leica, Biosystems Newcastle Ltd, UK), followed by AEC substrate kit (Vector Laboratories Inc. Burlingame, CA), according to the manufacturer's instruction. Hematoxylin and eosin (H&E) stain and immunohistochemistry study were performed as described previously48 (link). Isotype antibody was used as the staining negative control. Anti-Ki-67 and anti-caspase 3 antibodies were from Santa Cruz and Cell Signaling.

Free full text: Click here

Weng M.T., Tung T.H., Lee J.H., Wei S.C., Lin H.L., Huang Y.J., Wong J.M., Luo J, & Sheu J.C. (2015). Enhancer of rudimentary homolog regulates DNA damage response in hepatocellular carcinoma. Scientific Reports, 5, 9357.

Publication 2015

NoVo Link Polymer Detection System AEC substrate kit

Anti antibodies Antibody Caspase 3 Eosin Frozen sections Hematoxylin stain Immunohistochemistry Isotype Polymer Vector

Top 5 similar protocols

Variable analysis

independent variables

Staining method: NoVo Link Polymer Detection System

dependent variables

Immunohistochemistry analysis

control variables

Frozen sections (8 μm thick)
Hematoxylin and eosin (H&E) stain
Isotype antibody as staining negative control

controls

Isotype antibody as staining negative control

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!