NIH3T3 reporter cells were infected with MCMV, treated with IFNβ or IFNλ for 6 h and lysed for quantification of reporter gene expression (Le‐Trilling et al, 2018 (link)). Luciferase activity was measured according to the manufacturer's instructions (pjk) using a microplate multireader (Mithras2 LB 943; Berthold Technologies). Quantification of luminescence was performed by use of the microplate multireader Mithras2 LB 943 and MicroWin software (Berthold Technologies). The resulting data were analysed using GraphPad Prism software. The values are reported as mean ± standard deviation (SD) as described in the figure legends.
Protocol detail
Quantification of MCMV-Induced Luciferase
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Corresponding Organization :
Other organizations : University of Duisburg-Essen, Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Freie Universität Berlin, Technische Universität Berlin, Max Planck Institute for Molecular Genetics, Ruhr University Bochum, Universitätsklinikum Knappschaftskrankenhaus Bochum, University Medical Center Utrecht, Wellcome Centre for Cell Biology, University of Edinburgh
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Variable analysis
independent variables
- Treatment with IFNβ
- Treatment with IFNλ
- Reporter gene expression
- MCMV infection
- Measurement of luciferase activity using microplate multireader Mithras2 LB 943 and MicroWin software
- Positive control: Not specified
- Negative control: Not specified
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