The gene encoding the recombinant ferritin engineered from Helicobacter pylori non-heme ferritin and 2nd to 9th residues of bullfrog (Rana catesbeiana) ferritin lower subunit was a gift from Gary Nabel (44 (link)). Gene encoding Spike of SARS-CoV-2 (GenBank NC_0101080 ) codon-optimized for human codon usage (GenBank MC_0101081 ) was purchased from Genscript (pUC57–2019-nCoV-S). RBD was used to generate a fragment encoding RBD-SSGGASVLA linker-recombinant ferritin. For expression plasmid, a commercially available pFUSE vector (Invivogen) was engineered to replace human ferritin light chain gene promoter with SV40 promoter. Genes encoding the recombinant ferritin and the RBD-linker-ferritin fragment were cloned into the plasmid vector.
Protocol detail
Engineered Ferritin-RBD Fusion Protein
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2019 ncov
Codon
Codon usage
Ferritin
Ferritin light chain
Gene promoter
Genes
Helicobacter pylori
Heme ferritin
Human
Plasmid
Rana catesbeiana
Subunit
Sv40
Vector
Corresponding Organization : Cleveland Clinic Lerner College of Medicine
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Variable analysis
independent variables
- Gene encoding the recombinant ferritin engineered from Helicobacter pylori non-heme ferritin and 2nd to 9th residues of bullfrog (Rana catesbeiana) ferritin lower subunit
- Gene encoding Spike of SARS-CoV-2 (GenBank NC_0101080) codon-optimized for human codon usage (GenBank MC_0101081)
- Unclear from the provided information
- Unclear from the provided information
- Positive control: None mentioned
- Negative control: None mentioned
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