Rat MSC were isolated aseptically from bone marrow derived from the femurs of 6 to 10 week old male Wistar rats and characterized by flow cytometry analysis for selected surface markers per protocol as previously described [10 (link), 11 (link)]. rMSC cell migration was assessed using the QCM Chemotaxis Cell Migration Assay (8 μM, EMD Millipore, Billerica, MA) per manufacturer’s instructions. Monocyte chemoattractant protein-1 (MCP-1), stromal cell-derived factor 1 (SDF-1), platelet-derived growth factor-AA (PDGF-AA), and platelet-derived growth factor-BB (PDGF-BB) used to assess cell migration was obtained from Peprotech (Rocky Hill, NJ). rMSC at passage 2 to 4 were administered for transplantation.
Human MSC were derived and characterized by the NIH Bone Marrow Stromal Cell Transplantation Center [12 (link), 13 (link)] and cultured as previously described [14 (link)]. Superparamagnetic iron oxide nanoparticles (SPION) (ferumoxides (Feridex, Bayer AG, Germany) were complexed to the clinical transfection agent protamine sulfate as previously described [15 (link)]. hMSC at passage number less than 5 were magnetically labeled by co-culture with ferumoxides-protamine sulfate (Fe-Pro) in fresh media for 2 hours at 37°C as described [16 (link)].
Human MSC were derived and characterized by the NIH Bone Marrow Stromal Cell Transplantation Center [12 (link), 13 (link)] and cultured as previously described [14 (link)]. Superparamagnetic iron oxide nanoparticles (SPION) (ferumoxides (Feridex, Bayer AG, Germany) were complexed to the clinical transfection agent protamine sulfate as previously described [15 (link)]. hMSC at passage number less than 5 were magnetically labeled by co-culture with ferumoxides-protamine sulfate (Fe-Pro) in fresh media for 2 hours at 37°C as described [16 (link)].
Free full text: Click here
