Detailed procedures have been described previously (13 (link), 19 (link), 20 (link)). In brief, human RBCs were isolated from the whole blood of healthy volunteers according to a protocol approved by the Institutional Review Board of the Georgia Institute of Technology. RBCs were biotinylated, conjugated with streptavidin, and incubated with biotinylated pMHC for adhesion frequency assay with a micropipette. In the case of BFP experiments, silanized beads were covalently linked to streptavidin-maleimide (Sigma-Aldrich) then conjugated with subsaturating biotinylated pMHC.
Protocol detail
Biotinylated RBC Adhesion Assay
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Georgia Institute of Technology
Other organizations : Washington University in St. Louis, Emory University
Top 5 similar protocols
Variable analysis
independent variables
- Biotinylation of human RBCs
- Conjugation of RBCs with streptavidin
- Incubation of RBCs with biotinylated pMHC
- Covalent linking of silanized beads to streptavidin-maleimide
- Conjugation of beads with subsaturating biotinylated pMHC
- Adhesion frequency of RBCs in the micropipette assay
- Interactions between RBCs and pMHC-conjugated beads in the BFP experiments
- RBCs isolated from the whole blood of healthy volunteers
- Protocol approved by the Institutional Review Board of the Georgia Institute of Technology
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!