The Neisseria meningitidis FAM20 serogroup C strain and mutant deficient in PilC1 have been described previously [24 (link), 51 (link)]. The Neisseria meningitidis JB515 serogroup W strain and Neisseria gonorrhoeae MS11 strain have been described previously [24 (link), 52 (link)]. The strains were grown on GC agar (Acumedia) supplemented with 1% Kelloggs’ for 18 h at 37°C in a 5% CO2 environment. Antibiotics for selection of FAM20 mutant strains were used in following concentrations: tetracycline 1 μg/ml, kanamycin 50 μg/ml and spectinomycin 40 μg/ml.
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