Macrophages were grown to 90% confluency in 96-well plates (Costar) and exposed to 20 nm AgNPs, 110 nm AgNPs, or 20 nm Fe3O4 NPs at concentrations of 6.25 12.5, 25, or 50 μg/mL for 2h or 24h. The concentration range evaluated for cytotoxicity was selected based on previous in vitro experimentation of NPs [12 (link), 25 (link)]. Changes in cell viability were assessed using the MTS assay (Promega, Madison, WI) via manufacturer’s instructions using a spectrophotometer (BioTek Synergy HT, BioTek, Winooski, VT). A NP concentration of 25 μg/mL was selected for subsequent experiments due to limited induction of cytotoxicity at this concentration.
Macrophages were grown to 90% confluency in 96-well plates (Costar) and exposed to 20 nm AgNPs, 110 nm AgNPs, or 20 nm Fe3O4 NPs at a concentration of 25 μg/mL in serum-free media for 2 h and then treated with cholesterol (20 μg/mL) for 24 h. In a separate set of experiments macrophages were exposed to 20 nm AgNPs, 110 nm AgNPs, or 20 nm Fe3O4 NPs at a concentration of 25 μg/mL in serum-free media containing cholesterol (20 μg/mL) or without cholesterol present for 24 h. Changes in cell viability were again assessed using the MTS assay (Promega, Madison, WI) via manufacturer’s instructions using a spectrophotometer (BioTek Synergy HT, BioTek, Winooski, VT).
Macrophages were grown to 90% confluency in 96-well plates (Costar) and exposed to 20 nm AgNPs, 110 nm AgNPs, or 20 nm Fe3O4 NPs at a concentration of 25 μg/mL in serum-free media for 2 h and then treated with cholesterol (20 μg/mL) for 24 h. In a separate set of experiments macrophages were exposed to 20 nm AgNPs, 110 nm AgNPs, or 20 nm Fe3O4 NPs at a concentration of 25 μg/mL in serum-free media containing cholesterol (20 μg/mL) or without cholesterol present for 24 h. Changes in cell viability were again assessed using the MTS assay (Promega, Madison, WI) via manufacturer’s instructions using a spectrophotometer (BioTek Synergy HT, BioTek, Winooski, VT).
