Mitochondrial Protein Antibody Characterization

Antibodies used in this study were: mouse anti-c-Myc (Invitrogen, Product No. 132500, dilution 1:2,000 for immunoblotting, 1:50 for immunofluorescence), mouse anti-EF1a (Merck Millipore, Product No. 05-235, 1:10,000), goat anti-mouse Alexa Fluor 633 conjugated (Invitrogen, Product No. A21052, 1:1,000) and goat anti-rabbit FITC conjugated (Sigma, Product No. F0382, 1:1,000); polyclonal rabbit anti-ATOM40 (1:1,000 for both immunoblotting and immunofluorescence), anti-VDAC (1:1,000), anti-COXIV (1:1,000), anti-CYT C1 (1:1,000), anti-TIM9 (1:20) and anti-CYT C (1:100) were previously produced in our laboratory7 (link)15 (link)44 (link). Mouse anti-REAP1 (1:1,500)66 (link), rabbit anti-mtHSP70 (1:1,000)67 (link), rabbit anti-MCP5 (1:2,500)68 (link), rabbit anti-LIPDH (1:5,000)69 (link), rabbit anti-PEX14 (1:1,000)70 (link), rabbit anti-aldolase (1:50,000)71 (link), rabbit anti-GAPDH (1:50,000)72 (link), rabbit anti-G3PDH (1:1,000) and rabbit anti-GIM5 (1:10,000)37 (link) were kindly provided by L. Krauth-Siegel, S.H. Hajduk, R. Jensen, F. Voncken, P. Michels, A. Jardim and C. Clayton, respectively. Full scans for western blots are shown in Supplementary Fig. 8.

Free full text: Click here

Peikert C.D., Mani J., Morgenstern M., Käser S., Knapp B., Wenger C., Harsman A., Oeljeklaus S., Schneider A, & Warscheid B. (2017). Charting organellar importomes by quantitative mass spectrometry. Nature Communications, 8, 15272.

Publication 2017

mouse anti-c-Myc mouse anti-EF1a goat anti-rabbit FITC conjugated

Aldolase Antibodies Dilution Fitc Gapdh Goat Immunofluorescence Mouse Rabbit Scans Vdac 1 Western blots

Corresponding Organization : University of Freiburg

Other organizations : University of Bern

Top 5 similar protocols

Variable analysis

independent variables

Mouse anti-c-Myc (Invitrogen, Product No. 132500, dilution 1:2,000 for immunoblotting, 1:50 for immunofluorescence)
Mouse anti-EF1a (Merck Millipore, Product No. 05-235, 1:10,000)
Goat anti-mouse Alexa Fluor 633 conjugated (Invitrogen, Product No. A21052, 1:1,000)
Goat anti-rabbit FITC conjugated (Sigma, Product No. F0382, 1:1,000)
Polyclonal rabbit anti-ATOM40 (1:1,000 for both immunoblotting and immunofluorescence)
Anti-VDAC (1:1,000)
Anti-COXIV (1:1,000)
Anti-CYT C1 (1:1,000)
Anti-TIM9 (1:20)
Anti-CYT C (1:100)
Mouse anti-REAP1 (1:1,500)
Rabbit anti-mtHSP70 (1:1,000)
Rabbit anti-MCP5 (1:2,500)
Rabbit anti-LIPDH (1:5,000)
Rabbit anti-PEX14 (1:1,000)
Rabbit anti-aldolase (1:50,000)
Rabbit anti-GAPDH (1:50,000)
Rabbit anti-G3PDH (1:1,000)
Rabbit anti-GIM5 (1:10,000)

dependent variables

Protein expression levels

control variables

Full scans for western blots are shown in Supplementary Fig. 8

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!