Immunoprecipitation and western blotting were performed as described previously [9 (link), 15 (link)]. Membranes were probed with antibodies against BCAR4 (C78-I97, see above), EGFR (clone 2.1E1, Zytomed Systems, Berlin, Germany), ERBB2 (clone 4B5, Ventana, Tucson, AZ, U.S.A.), and β-actin (clone AC15, Acris, Hiddenhausen, Germany) and Anti-Flag M1 (Sigma, Zwijndrecht, the Netherlands).
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