Endothelial Barrier Integrity Assessment

Endothelial cells were seeded at 50,000 cells per insert well (0.4 μm pore size polyester membrane from Corning, Inc., Corning, NY, USA) and cultured for 1 to 3 days to allow the growth of a confluent monolayer. After the different groups received different treatments, the endothelial cell monolayers were treated with 100 ng/ml LPS for 6 h before testing for permeability. Paracellular and transcellular permeability were tested as described previously [9 (link)].

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Wang H., Zheng R., Chen Q., Shao J., Yu J, & Hu S. (2017). Mesenchymal stem cells microvesicles stabilize endothelial barrier function partly mediated by hepatocyte growth factor (HGF). Stem Cell Research & Therapy, 8, 211.

Publication 2017

polyester membrane

Cells Endothelial cell Membrane Permeability Polyester

Corresponding Organization : Yangzhou University

Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Sun Yat-sen University

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Variable analysis

independent variables

Different treatments received by the different groups

dependent variables

Paracellular permeability
Transcellular permeability

control variables

Endothelial cells seeded at 50,000 cells per insert well (0.4 μm pore size polyester membrane from Corning, Inc., Corning, NY, USA)
Endothelial cells cultured for 1 to 3 days to allow the growth of a confluent monolayer
Endothelial cell monolayers treated with 100 ng/ml LPS for 6 h before testing for permeability

controls

Positive control: Not specified
Negative control: Not specified

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