Immunofluorescence and Western Blot Analysis

The primary antibodies, including indoleamine 2, 3 dioxygenase1 (IDO1), ionized calcium binding adapter molecule 1 (Iba1), glial fibrillary acidic protein (GFAP), T-brain gene-2 (Tbr2), doublecortin (DCX), Prox1, act-casp3, PV, c-Fos, Calretinin, occluding, β-actin, toll-like receptor 4 (TLR4), and NF-κB, were purchased from Abcam, Invitrogen, Santa Cruz Biotechnology, and Cell Signaling Technology companies. The western blot and immunofluorescence procedures were performed following our previous protocols with minor modifications 26 (link). The modification is mainly the adjustment of the antibody dilution.

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Sun P., Wang M., Li Z., Wei J., Liu F., Zheng W., Zhu X., Chai X, & Zhao S. (2022). Eucommiae cortex polysaccharides mitigate obesogenic diet-induced cognitive and social dysfunction via modulation of gut microbiota and tryptophan metabolism. Theranostics, 12(8), 3637-3655.

Publication 2022

Iba1 Prox1 act-casp3 c-Fos Calretinin occluding β-actin toll-like receptor 4 (TLR4), NF-κB

Actin Antibodies Antibody Brain 2 C fos Calcium Calretinin Casp3 Dilution Gene Glial fibrillary acidic protein Immunofluorescence procedures Nf κb Toll like receptor 4 Western blot

Corresponding Organization :

Other organizations : Northwest A&F University, Xi'an Medical University

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Variable analysis

independent variables

Antibodies (IDO1, Iba1, GFAP, Tbr2, DCX, Prox1, act-casp3, PV, c-Fos, Calretinin, occludin, TLR4, NF-κB)

dependent variables

Protein expression levels of the antibodies

control variables

Western blot and immunofluorescence procedures (followed previous protocols with minor modifications)
Antibody dilution (adjusted)

positive controls

Not specified

negative controls

Not specified

Annotations

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