In vitro cultivation of Babesia ovata

Bovine blood was purchased from Japan Bio Serum (Tokyo, Japan) for the in vitro cultivation of the intraerythrocytic parasites, Babesia ovata (Miyake strain; Minami and Ishihara, 1980 ). After preparing red blood cells (RBCs), B. ovata was cultivated with RBCs in a sterilized mixture of GIT (Fujifilm Wako Pure Chemical, Osaka, Japan) and M199 media (Sigma-Aldrich, MO, USA) under a low-oxygen atmosphere, 5% O₂, 5% CO₂, and 90% N₂, at 37°C as described previously (Igarashi et al., 1994 (link); Maeda et al., 2016 (link)).

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Kuniyori M., Sato N., Yokoyama N., Kawazu S.I., Xuan X., Suzuki H., Fujisaki K, & Umemiya-Shirafuji R. (2022). Vitellogenin-2 Accumulation in the Fat Body and Hemolymph of Babesia-Infected Haemaphysalis longicornis Ticks. Frontiers in Cellular and Infection Microbiology, 12, 908142.

Publication 2022

M199 media

Atmosphere Babesia Blood Bovine Parasites Red blood cells Serum Strain

Corresponding Organization :

Other organizations : Obihiro University of Agriculture and Veterinary Medicine, National Agriculture and Food Research Organization

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Variable analysis

independent variables

Oxygen concentration (5% O2, 5% CO2, 90% N2)

dependent variables

Growth and cultivation of Babesia ovata parasites in red blood cells

control variables

Temperature (37°C)
Culture media (GIT and M199)
Blood source (Bovine blood from Japan Bio Serum)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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