Peptide Synthesis Reagents and Cell Culture Protocols

Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, IL), NovaBiochem (La Jolla, CA), or Anaspec (San Jose, CA). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, CA). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, MO). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, MA). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, CA).

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US11878046B2. Di-sulfide containing cell penetrating peptides and methods of making and using thereof (2024-01-23). Ohio State Innovation Foundation [US]. Inventors: Dehua Pei [US], Ziqing Qian [US].

Patent 2024

Cell culture media fetal bovine serum penicillin-streptomycin 0.25% trypsin-EDTA DPBS Methyl 3,5-dimethylbenzoiate N-bromosuccinimide diethyl phosphite 2,2′-dipyridyl disulfide Anti-GST-Tb streptavidin-d2 Luc)-HEK293 cell line One-Step™ luciferase assay system

Assay Cell Cell culture Culture media Disulfide Edta Fetal bovine serum Hek293 cell line Luciferase N bromosuccinimide Nf κb Penicillin Peptide synthesis Phosphite Rink amide resin Solvents Streptavidin Streptomycin Synthesis Trypsin

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

None explicitly mentioned

controls

No positive or negative controls mentioned

Annotations

Based on most similar protocols

All protocols use common reagents and cell lines from reputable commercial sources, ensuring consistent quality and minimizing batch-to-batch variations (Input, Protocol 1, Protocol 2, Protocol 3, Protocol 4, Protocol 5).

Multiple suppliers are used for peptide synthesis reagents, providing options to source materials and ensure availability (Input, Protocol 1, Protocol 3, Protocol 4, Protocol 5).

Protocols specify the use of specific mesh resins, resin loading capacities, and other key parameters for peptide synthesis to optimize yield and purity (Input, Protocol 1, Protocol 3, Protocol 4, Protocol 5).

Cell culture media, supplements, and antibiotics are consistently sourced from reputable suppliers, ensuring consistent cell growth conditions across experiments (Input, Protocol 1, Protocol 2, Protocol 3, Protocol 5).

Protocols specify the use of positive controls, such as antibodies, enzymes, and reporter cell lines, to validate assay performance and ensure reliable data interpretation (Input, Protocol 1, Protocol 3, Protocol 4).

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As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

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