DNA Extraction from Dried Blood Spots

All DBSs for the pfhrp2 and pfhrp3 studies were shipped to the Australian Defence Force Malaria and Infectious Disease Institute (ADFMIDI) where molecular testing was conducted. From each DBS sample, three discs of DBS were punched into 1.5-mL microfuge tubes. DNA was extracted using QIAamp DNA Mini Kits and a QIAcube Robot (QIAGEN, Crawley, UK) according to the manufacturer’s instructions. Samples were eluted into a volume of 100 µl with AE buffer. A P. falciparum-positive control DBS spot was extracted and processed in each run alongside samples. Details of the QIAamp DNA Mini Kits and a QIAcube Robot extraction method has been described and published elsewhere [12 (link), 17 (link), 25 (link), 36 (link), 37 (link)].

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Bosco A.B., Anderson K., Gresty K., Prosser C., Smith D., Nankabirwa J.I., Nsobya S., Yeka A., Opigo J., Gonahasa S., Namubiru R., Arinaitwe E., Mbaka P., Kissa J., Won S., Lee B., Lim C.S., Karamagi C., Cunningham J., Nakayaga J.K., Kamya M.R, & Cheng Q. (2020). Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019. Malaria Journal, 19, 300.

Publication 2020

QIAamp DNA Mini Kits QIAcube Robot

Buffer Infectious disease Malaria

Corresponding Organization : Makerere University

Other organizations : QIMR Berghofer Medical Research Institute, Malaria Consortium, Infectious Diseases Research Collaboration, World Health Organization - Uganda, Seoul National University, Korea University, World Health Organization

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Presence/absence of pfhrp2 and pfhrp3 genes in Plasmodium falciparum samples

control variables

None explicitly mentioned

controls

A P. falciparum-positive control DBS spot was extracted and processed in each run alongside samples.

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