Samples of organs and tissues were fixed in 10% neutral formalin solution for at least 48 h, then washed in running tap water, dehydrated in ascending alcohols, and embedded in paraffin. Paraffin sections 3–5 μm thick, stained with hematoxylin and eosin, were examined using conventional light microscopy on an AxioScopeA1 microscope (Carl Zeiss, Germany). To assess the severity of histological changes, a scoring scale was used (Breljak et al., 2015 (link)).
Organ and tissue samples fixed in 10% neutral formalin solution for at least 48 h
Organ and tissue samples washed in running tap water
Organ and tissue samples dehydrated in ascending alcohols
Organ and tissue samples embedded in paraffin
Paraffin sections 3–5 μm thick
Paraffin sections stained with hematoxylin and eosin
Examination using conventional light microscopy on an AxioScopeA1 microscope (Carl Zeiss, Germany)
controls
Positive control: Not specified
Negative control: Not specified
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