Quantification of Myokines in Exercise Intervention

Myokines were measured in serum samples collected from the participants at baseline and follow-up of the exercise intervention, as well as in the cell-culture supernatants of myotubes derived from biopsy samples obtained at baseline and follow-up timepoints. Myokine quantifications were performed using a single-plex assay (R-plex; SPARC) and a multiplex assay (U-plex; BDNF, FGF21, IL-6, IL-8, IL-10, IL-13, IL-15, and IL-18) from Meso Scale Discovery (Rockville, MD, USA) following the manufacturer’s instructions and as previously published [68 (link),69 (link)]. All antibodies with the exception of SPARC were validated for target specificity. More information about specificity tests can be found in the datasheet of the U-plex kits (https://www.mesoscale.com/ (accessed on 21 March 2024)).