Mouse embryonic stem cell lines for imaging the Pou5f1 and Nanog loci were derived from Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male – species/sex verified by karyotyping, no additional cell line authentification performed). OMG mESCs39 (link) contain a 24 × MS2 cassette integrated in the 3′-UTR of one of the two Pou5f1 alleles57 (link) and also stably express MCP-mNeonGreen39 (link). NMG mESCs contain 24 × MS2 cassettes integrated in the 3′-UTR of both Nanog alleles and also stably express MCP-mNeonGreen58 (link). NMG SNAP-Brd4 clone 7 cells also contain bi-allelic integrations of SNAP-tag at the Brd4 locus39 (link). mESC lines for imaging the Sox2 locus were derived from B6 albino mESCs (B6(Cg)-Tyrc-2J/J, Jackson Lab 000058; male - species/sex verified by karyotyping, no additional cell line authentification performed). All cell lines tested negative for mycoplasma.
Protocol detail
Mouse Embryonic Stem Cell Lines for Imaging
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Memorial Sloan Kettering Cancer Center, Hiroshima University
Top 5 similar protocols
Variable analysis
independent variables
- Integration of 24 × MS2 cassette in the 3'-UTR of one of the two Pou5f1 alleles in OMG mESCs
- Integration of 24 × MS2 cassettes in the 3'-UTR of both Nanog alleles in NMG mESCs
- Integration of SNAP-tag at the Brd4 locus in NMG SNAP-Brd4 clone 7 cells
- Expression of MCP-mNeonGreen in OMG mESCs and NMG mESCs
- Imaging of Pou5f1 and Nanog loci in respective cell lines
- Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male) used as the parental cell line for OMG and NMG mESCs
- B6 albino mESCs (B6(Cg)-Tyrc-2J/J, Jackson Lab 000058; male) used as the parental cell line for Sox2 imaging
- All cell lines tested negative for mycoplasma
- Not specified
- Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!