Histological Profiling of Synovial Tissues

Formalin-fixed, paraffin-embedded synovial tissues of RA or OA patients were cut, put on slides and stained with hematoxylin/eosin. 36 synovial tissues from hands (22 from joint replacement and 14 from synovial biopsies) and 27 from knee (18 from joint replacement and 9 from synovial biopsies) were assessed. Synovitis score was assessed by evaluation of the thickness of the lining cell layer, the cellular density of synovial stroma and leukocyte infiltration as described by Krenn et al.^{30 (link)}. Vascularization was assessed by counting the amount of CD31+ cells (ab28364, Abcam; dilution: 1:50) on 5 consecutive pictures on 20× objective. Synovial tissue were stained with CD3 (ab16669, Abcam; dilution: 1:200), CD20 (M0755, Agilent/Dako; dilution: 1:50), CD138 (Clone MI15, Agilent/Dako; dilution: 1:50) and CD68 (M0814, Agilent/Dako; dilution: 1:50), to stratify them into lymphoid, myeloid and fibroid histological patterns according to previously published histological features^{7 (link),9 (link),30 (link)}.

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Elhai M., Micheroli R., Houtman M., Mirrahimi M., Moser L., Pauli C., Bürki K., Laimbacher A., Kania G., Klein K., Schätzle P., Frank Bertoncelj M., Edalat S.G., Keusch L., Khmelevskaya A., Toitou M., Geiss C., Rauer T., Sakkou M., Kollias G., Armaka M., Distler O, & Ospelt C. (2023). The long non-coding RNA HOTAIR contributes to joint-specific gene expression in rheumatoid arthritis. Nature Communications, 14, 8172.

Publication 2023

ab28364 ab16669 M0755 Clone MI15 M0814

Corresponding Organization :

Other organizations : University Hospital of Zurich, University of Zurich, Alexander Fleming Biomedical Sciences Research Center

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Variable analysis

independent variables

Tissue type (rheumatoid arthritis (RA) or osteoarthritis (OA))
Tissue source (joint replacement or synovial biopsy)

dependent variables

Synovitis score (based on the thickness of the lining cell layer, the cellular density of synovial stroma, and leukocyte infiltration)
Vascularization (measured by counting the amount of CD31+ cells)

control variables

Formalin-fixed, paraffin-embedded synovial tissues
Staining with hematoxylin/eosin
Immunohistochemical staining with CD3, CD20, CD138, and CD68

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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