To monitor the uptake of EVAvo by peritoneal macrophages, EVAvo was labelled with Exo‐GLOW Membrane EV Labeling Kit (System Biosciences) per the manufacturer's instructions.
46 (link) Briefly, EVAvo (300 μg) was mixed with the Exoglow fluorescent dye and incubated at RT for 30 min in the dark. We used 10 K MWCO buffer exchange tubes and centrifuged samples at 12000 g for 10 min to remove free unlabeled dye. Labelled EVAvo (2 or 10 μg/mL) was then added to macrophages and their uptake by the cells at different time points was visualized by fluorescence microscopy (Zeiss Axio Observer, White Plains, NY).
46 (link) Briefly, EVAvo (300 μg) was mixed with the Exoglow fluorescent dye and incubated at RT for 30 min in the dark. We used 10 K MWCO buffer exchange tubes and centrifuged samples at 12000 g for 10 min to remove free unlabeled dye. Labelled EVAvo (2 or 10 μg/mL) was then added to macrophages and their uptake by the cells at different time points was visualized by fluorescence microscopy (Zeiss Axio Observer, White Plains, NY).
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