Cancer Cell Lines and Nanoparticle Characterization

The human cancer cell lines Detroit 562 (#CCL-138, pharyngeal carcinoma), FaDu (#HTB-43, squamous cell pharyngeal carcinoma), HT1080 (#CCL-121, fibrosarcoma), MIA PaCa-2 (#CRL-1420, pancreas carcinoma), NCI-H460 (#HTB-177, large cell lung carcinoma) and T98G (#CRL-1690, glioblastoma) were purchased from ATCC. The human cancer cell lines CAL-33 (#ACC 447, tongue squamous cell carcinoma), DU-145 (#ACC 261, prostate carcinoma), HEP-3B (#ACC 93, hepatocellular carcinoma), LNCaP (#ACC 256, prostate carcinoma) and PC-3 (#ACC 465, prostate adenocarcinoma) were purchased from DSMZ. The human cancer cell lines MDA-MB-231-luc-D3-H2LN (breast adenocarcinoma) and NCI-H460-Luc2 (large cell lung carcinoma) were purchased from Caliper. Cells were cultivated according to manufacturer’s recommendations and were controlled for mycoplasma by an independent laboratory (Clean Cells). NBTXR3 (Nanobiotix) is a sterile aqueous suspension of functionalized crystal hafnium oxide (HfO₂) spherical nanoparticles with a size centered on 50nm, determined by dynamic light scattering technique (Zetasizer NanoZS, Malvern Instruments Ltd, Worcestershire, UK), bearing a marked negative surface charge (–50mV) in aqueous solution at pH 6–8,2 (link),4 (link) estimated by zeta potential analysis (Zetasizer NanoZS). Cisplatin (CDDP) was obtained from Sigma Aldrich.

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Zhang P., Marill J., Darmon A., Mohamed Anesary N., Lu B, & Paris S. (2021). NBTXR3 Radiotherapy-Activated Functionalized Hafnium Oxide Nanoparticles Show Efficient Antitumor Effects Across a Large Panel of Human Cancer Models. International Journal of Nanomedicine, 16, 2761-2773.

Publication 2021

Detroit 562 HT1080 MIA PaCa-2 NCI-H460 CAL-33 DU-145 HEP-3B LNCaP Zetasizer NanoZS Cisplatin (CDDP)

Adenocarcinoma Breast Cancer Cddp Cell lines Cells Fibrosarcoma Glioblastoma Hafnium oxide Hepatocellular carcinoma Human Large cell carcinoma Lung Mycoplasma Pancreas carcinoma Pharyngeal Pharyngeal carcinoma Prostate Prostate carcinoma Squamous cell carcinoma Sterile Tongue

Corresponding Organization : Nanobiotix (France)

Other organizations : Thomas Jefferson University

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Variable analysis

independent variables

NBTXR3 (Nanobiotix)
Cisplatin (CDDP)

dependent variables

Cell viability/growth
Mycoplasma contamination

control variables

Cell culture conditions (according to manufacturer's recommendations)
Cell lines (human cancer cell lines from ATCC, DSMZ, and Caliper)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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