The level of the 9S rRNA precursor of the p5S transcript was determined by qPCR as described above for the polar and non-polar mRNAs with two exceptions. First, cells were grown to the mid-exponential phase and then transferred to either permissive (30 °C) or restrictive (42 °C or 43 °C) temperature for 1 h of additional growth. Second, expression was normalized using gyrA mRNA as a reference.
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