N-(2-fluorphenyl)-6-chloro-4-hydroxy-2-quinolone-3-carboxamide (R19) was synthesized and characterized as previously described [9 (link)]. Pluronic P123 (P123), TPGS, and Nile red (NR) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline solution (PBS, 10×, pH 7.4), potassium bromide (KBr), and dimethyl sulfoxide (DMSO) were procured from Fisher Chemicals (Pittsburgh, PA, USA). Spectra/Por dialysis membranes with 3500 and 12,000–14,000 Da molecular weight cut-offs (MWCO) were obtained from Repligen (Waltham, MA, USA). Ultrapure water was prepared using a Millipore Direct-Q 5UV system (EMD Millipore, Burlington, MA, USA).
Free full text: Click here
