DNA from bacterial isolates was extracted using the NEB Monarch Purification Kit (New England Biolabs, Ipswich, MA, USA) and sequenced on a PacBio® Sequel IIe system (Pacific Biosciences, Menlo Park, CA, USA) as described previously (Effelsberg et al., 2021 (link)). A de novo assembly approach was used for the raw reads and analyzed using the SMRT® Link software suite v. 10 or v. 11 with default parameters.
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