Immunostaining of Cell-Based Organoids

Cells were seeded on Matrigel (Growth Factor Reduced Matrigel, BD Biosciences, Franklin Lakes, NJ, USA) using 2% Matrigel DMEM media supplemented with 10% fetal bovine serum and penicillin/streptomycin. Immunostaining with cell-based organoids was performed following a previously published protocol.^{36 (link)} Primary antibodies used were anti-E-cadherin (BD Bioscience, 610181) and anti-F-actin (Thermo Fisher, A12381). Images were acquired using a Zeiss LSM 510 microscope and analyzed with ImageJ software.

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Jung H.Y., Lee D.K., Lee M., Choi S.H., Park J.D., Ko E.S., Lee J., Park K.S, & Jung H.Y. (2023). ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer. Oncoimmunology, 12(1), 2190671.

Publication 2023

Growth Factor Reduced Matrigel anti-E-cadherin anti-F-actin LSM 510 microscope

Antibodies Cadherin Cell F actin Fetal bovine serum Growth factor Matrigel Microscope Organoids Penicillin Streptomycin

Corresponding Organization : CHA University

Other organizations : Korea University, Korea Institute of Radiological and Medical Sciences

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Variable analysis

independent variables

Matrigel (Growth Factor Reduced Matrigel, BD Biosciences, Franklin Lakes, NJ, USA)

dependent variables

Immunostaining with cell-based organoids
Images acquired using a Zeiss LSM 510 microscope and analyzed with ImageJ software

control variables

2% Matrigel DMEM media supplemented with 10% fetal bovine serum and penicillin/streptomycin
Previously published protocol for immunostaining ^{36 (link)}
Primary antibodies used: anti-E-cadherin (BD Bioscience, 610181) and anti-F-actin (Thermo Fisher, A12381)

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