Immunofluorescence was conducted as described previously (Bermudez-Hernandez et al., 2017 (link)). Free-floating sections were washed in 0.1 M TB, incubated in blocking serum for 1 h (10% goat serum, 0.025% (v/v) Triton X-100 and 0.005% (v/v) bovine serum albumin in 0.1 M Tris buffer), followed by washes in 0.1 M TB. Next, sections were incubated overnight at 4°C with a primary antibody (Rabbit polyclonal anti-mCherry 1:500, Cat# ab167453, RRID:AB_2571870; Abcam, Cambridge, MA) diluted in blocking serum. The next day, sections were washed in 0.1 M TB, followed by a 2 h-long incubation in secondary antibody (anti-rabbit IgG made in goat, Alexa Fluor 568; 1:1000; Cat# A11036, RRID:AB_10563566, Thermo Fisher Scientific) diluted in blocking serum. Sections were then washed in 0.1 M TB. Sections were mounted on gelatin-coated slides and coverslipped with Citifluor™ AF1 mounting solution (Cat# 17970–25, Electron Microscopy Sciences). Sections were viewed with a confocal microscope (LSM 510 Meta). Photography was conducted as described above for FJ.