Human glioblastoma cell lines (U87, U251, LN229, A172, U118, and LN18)
were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and
were authenticated in 2017 by comparison of STR profiles to the ATCC public
dataset. Gli36 was provided by Dr. Khalid Shah at Massachusetts General
Hospital, Boston, MA, in 2014. Normal human astrocytes (NHA) were purchased from
ScienCell in 2009, and used before passage 10. Glioblastoma cell lines and NHA
were maintained in Dulbecco’s modified Eagle medium (DMEM) with 4.5 g/L
glucose, L-glutamine and sodium pyruvate supplemented with 10% fetal bovine
serum and 1% penicillin/Streptomycin/Amphotericin. Patient-derived glioma
neurosphere lines (MGG4, MGG6, MGG8, MGG18, MGG23, MGG75, and MGG152) were
established and cultured in serum-free neural cell medium as described
previously (20 (link)–22 (link)). All the glioma cell lines were confirmed to be
mycoplasma-free using LookOut Mycoplasma PCR Detection Kit from Sigma in
2016–2017. Volasertib (23 (link)),
Irinotecan, KU-55933 (24 (link)), VE-821 (25 (link)), Alisertib (26 (link)), Barasertib (27 (link)), MK8776 (28 (link)), NU7441
(29 (link)), Palbociclib, Carboplatin, and
Imatinib were purchased from SelleckChem. GSK461364 (30 , 31 (link)) was
from APExBIO and Temozolomide, Etoposide, and Ex527 (32 (link)) were from Sigma-Aldrich.
were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and
were authenticated in 2017 by comparison of STR profiles to the ATCC public
dataset. Gli36 was provided by Dr. Khalid Shah at Massachusetts General
Hospital, Boston, MA, in 2014. Normal human astrocytes (NHA) were purchased from
ScienCell in 2009, and used before passage 10. Glioblastoma cell lines and NHA
were maintained in Dulbecco’s modified Eagle medium (DMEM) with 4.5 g/L
glucose, L-glutamine and sodium pyruvate supplemented with 10% fetal bovine
serum and 1% penicillin/Streptomycin/Amphotericin. Patient-derived glioma
neurosphere lines (MGG4, MGG6, MGG8, MGG18, MGG23, MGG75, and MGG152) were
established and cultured in serum-free neural cell medium as described
previously (20 (link)–22 (link)). All the glioma cell lines were confirmed to be
mycoplasma-free using LookOut Mycoplasma PCR Detection Kit from Sigma in
2016–2017. Volasertib (23 (link)),
Irinotecan, KU-55933 (24 (link)), VE-821 (25 (link)), Alisertib (26 (link)), Barasertib (27 (link)), MK8776 (28 (link)), NU7441
(29 (link)), Palbociclib, Carboplatin, and
Imatinib were purchased from SelleckChem. GSK461364 (30 , 31 (link)) was
from APExBIO and Temozolomide, Etoposide, and Ex527 (32 (link)) were from Sigma-Aldrich.
