Primary human dermal fibroblasts were maintained in Dulbecco’s Modified Eagle Medium (DMEM) cell culture medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C, 5% CO2 and 95% humidity (standard cell culture conditions). Cell culture medium and supplements were all purchased from Gibco, Invitrogen, Thermo Fisher Scientific Inc, Dreieich, Germany.
For all experiments, the 3D collagen matrices were placed into four well-plates (Thermo Fisher Scientific Inc, Dreieich, Germany). 1 × 105 fibroblast cells were seeded onto 3D collagen matrices and kept at standard cell culture conditions for 2 h to allow for cell attachment. Afterwards, the cell culture medium was removed and biocompatible microvessels were placed onto the well plate, as previously described [37 (link)]. Subsequently, fresh cell culture medium was added into the microvessel. For fibroblast differentiation, cell culture medium was supplemented with 10 ng/mL TGF- β1(Biolegend, San Diego, CA, USA) [21 (link)]. Cells were then cultured for 3 days at 1g or sµG conditions in an incubator at standard cell culture conditions.
For all experiments, the 3D collagen matrices were placed into four well-plates (Thermo Fisher Scientific Inc, Dreieich, Germany). 1 × 105 fibroblast cells were seeded onto 3D collagen matrices and kept at standard cell culture conditions for 2 h to allow for cell attachment. Afterwards, the cell culture medium was removed and biocompatible microvessels were placed onto the well plate, as previously described [37 (link)]. Subsequently, fresh cell culture medium was added into the microvessel. For fibroblast differentiation, cell culture medium was supplemented with 10 ng/mL TGF- β1(Biolegend, San Diego, CA, USA) [21 (link)]. Cells were then cultured for 3 days at 1g or sµG conditions in an incubator at standard cell culture conditions.
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