Cytokine Profiling in Ovarian Cancer

For cytokine array analysis, frozen cell-free ascites fluid and peripheral blood plasma samples were submitted to Eve Technologies for analysis on their Human Cytokine/Chemokine Array 48-Plex. Forty-eight cytokines, chemokines, and growth factors were analyzed in duplicate from 100 μL of sample. Human M-CSF and VEGF-A levels were measured in patient and PDX cell-free ascites fluid using the Human M-CSF ELISA Kit or Human VEGF-A ELISA kit, respectively (RayBiotech). huPDX cytokine levels were compared with published LINCOplex microarray data from paired ascites and plasma samples from ovarian cancer (29 (link)). Macrophage-derived chemokine (MDC) values were compared with published data from 93 patients with ovarian cancer (30 (link)). For uniformity, average and SEM for MDC levels were estimated from the listed median and interquartile range using an online estimator (31 (link)).

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Steinkamp M.P., Lagutina I., Brayer K.J., Schultz F., Burke D., Pankratz V.S., Adams S.F., Hudson L.G., Ness S.A, & Wandinger-Ness A. (2023). Humanized Patient-derived Xenograft Models of Disseminated Ovarian Cancer Recapitulate Key Aspects of the Tumor Immune Environment within the Peritoneal Cavity. Cancer Research Communications, 3(2), 309-324.

Publication 2023

Human VEGF-A ELISA kit

Ascites Ascites fluid Cell Chemokines Cytokine Elisa Frozen Growth factors Human M csf Macrophage derived chemokine Microarray Ovarian cancer Patients Plasma Vegf

Corresponding Organization : New Mexico Cancer Center

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Variable analysis

independent variables

Cell-free ascites fluid and peripheral blood plasma samples from patient and PDX

dependent variables

Levels of 48 cytokines, chemokines, and growth factors
Levels of M-CSF and VEGF-A in patient and PDX cell-free ascites fluid

control variables

Volume of sample (100 μL) used for cytokine array analysis

controls

Positive control: Published LINCOplex microarray data from paired ascites and plasma samples from ovarian cancer patients
Positive control: Published data from 93 patients with ovarian cancer for macrophage-derived chemokine (MDC) levels

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