Gene expression of the complete denitrifier P. fluorescens JL1 in response to hyphal exudates was determined. Citrate and malate were selected as carbon sources based on the results from the serum bottle assay. Glucose was used as the control. The experimental design was the same as in the serum bottle assay. At 0.5, 1, 2, 3, and 6 h, total RNA were extracted and relative changes in nirS and nosZ genes normalized by the 2−ΔΔCt method [35 (link)]. Details are shown in the Supplementary Information.
Li X., Zhao R., Li D., Wang G., Bei S., Ju X., An R., Li L., Kuyper T.W., Christie P., Bender F.S., Veen C., van der Heijden M.G., van der Putten W.H., Zhang F., Butterbach-Bahl K, & Zhang J. (2023). Mycorrhiza-mediated recruitment of complete denitrifying Pseudomonas reduces N2O emissions from soil. Microbiome, 11, 45.
Other organizations :
China Agricultural University, Hainan University, Wageningen University & Research, University of Zurich, Netherlands Institute of Ecology, Karlsruhe Institute of Technology
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