Cell lines are maintained as part of the cell line repository within the Division of Reproductive Sciences at Duke University. For the details, please refer to (18 (link)). STR profiling was performed at the Duke University DNA Analysis Facility each time when frozen stocks are prepared; they were last genotyped on 6/5/18. Mycoplasma testing were conducted at the Cell Culture Facility, Duke Universityon 6/5/18. For nutrigenetic screens, all OVCA cells were cultured in a humidified incubator at 37°C and 5% CO2 using custom-made Dulbecco’s Modified Eagle Medium (11995-DMEM, ThermoFisher Scientific) with 10% heat-inactivated and dialyzed Fetal Bovine Serum (HyCloneTM FBS, GE Healthcare Life Sciences #SH30070.03HI) with the indicated amino acid removed as previously described (20 (link),21 (link)). Transfections were performed according to the manufacturer’s instructions with TransIT-LT1 transfection reagent (Mirus Bio) or RNAiMax transfection reagent (ThermoFisher Scientific).
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OVCA Cell Line Maintenance and Transfection
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Corresponding Organization : Duke University
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Variable analysis
independent variables
- Amino acid removal
- Nutrigenetic screen outcomes
- Cell culture conditions (humidified incubator at 37°C and 5% CO2)
- Cell culture medium (custom-made Dulbecco's Modified Eagle Medium with 10% heat-inactivated and dialyzed Fetal Bovine Serum)
- Cell line (OVCA cells)
- STR profiling and mycoplasma testing
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