Antibody Production and Characterization

Rabbit anti-Eap polyclonal antibody was developed by Scrum (Tokyo, Japan)^{25 (link)}. Rabbit anti-SasG polyclonal antibody⁹ and mouse anti-SasG polyclonal antibodies were developed by Eurofins Genomics (Tokyo, Japan). Rabbit anti-Curli antibody was developed by Scrum (Tokyo, Japan), and mouse anti-dsDNA monoclonal antibody was purchased from Abcam (Cambridge, MA, USA)^{33 (link)}. Alexa 405-conjugated anti-mouse IgG, Alexa 488-conjugated goat anti-rabbit IgG, Alexa 647-conjugated goat anti-rabbit IgG, and Alexa 647-conjugated goat anti-mouse IgG were purchased from Thermo Fisher Scientific. Alexa Fluor 647-conjugated anti-mouse lymphocyte antigen 6 complex locus G antibody (anti-Ly-6G-Alexa 647) was purchased from BioLegend Japan, Inc. (Tokyo, Japan).