The assay was performed exactly as in the standard variant with the following modifications. After 1 h incubation with the virus, we did not remove the inoculum. We added 100 μl/well of Avicel overlay media and mixed the plate either by tapping with hand or using a plate mixer. The media included increased amounts of trypsin (1.5 μg/ml) to compensate for the dilution of the overlay with the virus-containing material present in the wells.
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