The whole blood samples were collected from IE patients in the mean time with blood culture, and then about 300ul plasma (the whole blood sample centrifugated at 4°C) of each patient was used to extracted DNA by TIANamp Micro DNA Kit following the manufacturer’s instructions. DNA libraries were quality controlled using an Agilent 2100 Bioanalyzer (Agilent, USA) and an ABI StepOnePlus real-time polymerase chain reaction (PCR) system, and qualified libraries were sequenced on a NextSeq 550Dx platform (illumina, USA) using a 75 bp sequencing read length as previously described (Zhao et al., 2022 (link)). After the low-quality reads were removed, the clean reads were aligned to human genome to remove host sequence reads. All selected reads were mapped to the commercial pathogen database to annotate and analyze. Template free control (NTC) was obtained by repeated DNA extraction and sequencing using blank tubes filled with UltraPure Dnase/Rnase-free distilled water.
The data that support the findings of this study have been deposited into EMBL database with accession number PRJEB57970.
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