Transcriptional Activator Engineering in Chrysanthemum

CmWRKY8-1 was fused to the VP64 (4 × VP16) protein to produce the transcriptional activator R4-FVuv-CmWRKY8-1. The vector was introduced into Agrobacterium tumefaciens strain EHA105 and ‘Jinba’ was transformed by Agrobacterium-mediated transformation [51 (link)]. Transgenic chrysanthemums were primed with vector primers and CmWRKY8-1 specific primers (Table S2) for DNA-level identification. qRT-PCR analysis at the RNA level was performed using CmWRKY8-1 quantitative primers.

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Miao W., Ge L., Wang Y., Li S., Sun D., Liu Y., Guan Z., Chen S., Fang W., Chen F, & Zhao S. (2023). Overexpression of CmWRKY8-1–VP64 Fusion Protein Reduces Resistance in Response to Fusarium oxysporum by Modulating the Salicylic Acid Signaling Pathway in Chrysanthemum morifolium. International Journal of Molecular Sciences, 24(4), 3499.

Publication 2023

Agrobacterium Agrobacterium tumefaciens Chrysanthemums Primers Strain Transcriptional Transgenic Vector Vp16 protein

Corresponding Organization : Ministry of Agriculture and Rural Affairs

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Variable analysis

independent variables

CmWRKY8-1 fused to VP64 (4 × VP16) protein to produce the transcriptional activator R4-FVuv-CmWRKY8-1

dependent variables

Transgenic chrysanthemum identification at the DNA level
CmWRKY8-1 expression at the RNA level

control variables

Agrobacterium tumefaciens strain EHA105 used for transformation
'Jinba' cultivar used for transformation

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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