Pluripotency and Cardiac Differentiation Markers

Expression of pluripotent, gastrulation, and cardiac markers was detected by RT-PCR.37 (link),38 (link) Mouse Gapdh (4352932E; Applied Biosystems) was used as control. Analyzed genes included Sox2 (Mm00488369_s1), Oct4 (Mm00658129_gH), Fgf4 (Mm00438917_m1), Gsc (Mm00650681_g1), Sox17 (Mm00488363_m1), Mesp2 (Mm00655937_m1), Tbx5 (Mm00803521_m1), Nkx2.5 (Mm00657783_m1) and Mef2c (Mm01340839_m1; Applied Biosystems).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Martinez-Fernandez A., Nelson T.J., Yamada S., Reyes S., Alekseev A.E., Perez-Terzic C., Ikeda Y, & Terzic A. (2009). iPS Programmed Without c-MYC Yield Proficient Cardiogenesis for Functional Heart Chimerism. Circulation research, 105(7), 648-656.

Publication 2009

Cardiac Fgf4 Gapdh Gastrulation Genes Mouse Oct4 Rt pcr Sox2

Top 5 similar protocols

Protocol cited in 5 other protocols

Variable analysis

independent variables

Expression of pluripotent, gastrulation, and cardiac markers

dependent variables

Expression of genes including Sox2, Oct4, Fgf4, Gsc, Sox17, Mesp2, Tbx5, Nkx2.5, and Mef2c

control variables

Mouse Gapdh (4352932E; Applied Biosystems)

controls

Positive control: Mouse Gapdh (4352932E; Applied Biosystems)
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!