Phenotypic Characterization of Human Adipose-Derived Stem Cells

Cells were harvested, washed and centrifuged for 5 min at 300 g. Pellets were resuspended in sterile complete media, and cell counts were determined. Approximately 500,000 cells were used for each reaction, including different cell types for comparison purposes (fibroblasts and hASCs). Cells were recorded on a BD LSR II flow cytometer (BD Biosciences, Oxford, UK) using BD FACSDiva software, and data were analyzed using FlowJo software (TreeStar., Ashland, OR, USA) [19 (link)]. The following panel of antibodes were used for hASC characterization: APC mouse anti-human CD45 (BD Biosciences catalog no #555485), PE mouse anti-human CD34 (BD Horizon, BD Biosciences, catalog no #562577), PE mouse anti-human CD73 (BD Biosciences catalog no #550257), PE mouse anti-human CD90 (Biosciences catalog no #555596) and PE mouse anti-human CD-105 (BD Biosciences catalog no #560839). The percentage of fluorescence in hASCs was determined.