Cell Culture Conditions for BCC and Other Cells

ASZ001 and BSZ001 BCC cells were cultured in 154CF media (Life
Technologies) supplemented with 2% chelated fetal bovine serum and
0.05mM CaCl₂. Experiments carried out using low serum conditions
contained 154CF media containing 0.2% chelated FBS and 0.5nM
CaCl₂. NIH-3T3, HaCaT, and HEK-293T cells were cultured in DMEM
supplemented with 10% fetal bovine serum. Hedgehog induction experiments
carried out using low-serum DMEM containing 0.5% FBS. UW-BCC1 cells
(human BCC cells) were isolated from a patient with superficial BCC. Cells were
cultured as described previously^{55 (link)}.

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Whitson R.J., Lee A., Urman N.M., Mirza A., Yao C.Y., Brown A.S., Li J.R., Shankar G., Fry M.A., Atwood S.X., Hollmig S.T., Aasi S.Z., Sarin K.Y., Epstein EH J.r., Tang J.Y, & Oro A.E. (2018). Non-canonical hedgehog pathway activation by MKL1/SRF promotes drug-resistance in basal cell carcinomas. Nature medicine, 24(3), 271-281.

Publication 2018

154CF media

293t cells Cells Cultured media Hedgehog Human Low serum media Nih 3t3 Patient Serum

Corresponding Organization : Stanford University

Other organizations : University of California, Irvine

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Variable analysis

independent variables

Serum concentration (2% chelated FBS vs. 0.2% chelated FBS)
Calcium chloride concentration (0.05 mM vs. 0.5 nM)

dependent variables

Cell growth/proliferation of ASZ001 and BSZ001 BCC cells
Hedgehog signaling in NIH-3T3, HaCaT, and HEK-293T cells

control variables

Cell lines used (ASZ001, BSZ001, NIH-3T3, HaCaT, HEK-293T, UW-BCC1)
Media used (154CF media, DMEM)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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