Identification of IDH1 and IDH2 Mutations in FFPE Tumor Tissue

Tumor DNA extracted from FFPE tumor tissue (FFPE Tissue Kit, Qiagen S.r.l., Milano, Italy) was amplified using specific primers for exon 4 of IDH1 and IDH2 genes. (IDH1: forward primer 5’-TGTAAAACGACGGCCAGTGGATGCTGCAGAAGCTATAA-3’; reverse primer 5’-CAGGAAACAGCTATGACCTTCATACCTTGCTTAATGGG-3’. IDH2: forward primer 5’-TGTAAAACGACGGCCAGTAATTTTAGGACCCCCGTCTG-3’; reverse primer 5’-CAGGAAACAGCTATGACCGGGGTGAAGACCATTTTGAA-3’). Codon 100 and 132 of IDH1 and codon 140 and 172 of IDH2 were then analyzed by Sanger sequencing method [14 (link)].

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Pagano C., Navarra G., Coppola L., Avilia G., Pastorino O., Della Monica R., Buonaiuto M., Torelli G., Caiazzo P., Bifulco M, & Laezza C. (2022). N6-isopentenyladenosine induces cell death through necroptosis in human glioblastoma cells. Cell Death Discovery, 8, 173.

Publication 2022

FFPE Tissue Kit

Codon Exon Genes Idh2 Primer Tissue Tumor Tumor dna

Corresponding Organization : National Research Council

Other organizations : Ospedali Riuniti San Giovanni di Dio e Ruggi d'Aragona, Ospedale Antonio Cardarelli

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Variable analysis

independent variables

Primers for exon 4 of IDH1 and IDH2 genes

dependent variables

Mutations in codon 100 and 132 of IDH1 and codon 140 and 172 of IDH2

control variables

FFPE tumor tissue samples
Qiagen S.r.l., Milano, Italy FFPE Tissue Kit

controls

No positive or negative controls were explicitly mentioned.

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