Recombinant Production of LukS-PV Toxin

The sequence of LukS-PV was amplified from PVL-positive Staphylococcus aureus isolates by PCR, and recombinant LukS-PV was generated as described previously.16 (link) Recombinant LukS-PV was purified using a His-Bind Purification Kit (Millipore, USA) and quantified using a BCA Kit (Beyotime, China).

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Ma F., Wang Z., Qiang Y., Xu L., Ding P., Wang Y, & Ma X. (2020). LukS-PV Inhibits Hepatocellular Carcinoma Cells Migration via the TNNC1/PI3K/AKT Axis. OncoTargets and therapy, 13, 10221-10230.

Publication 2020

His-Bind Purification Kit BCA Kit

Staphylococcus aureus

Corresponding Organization : University of Science and Technology of China

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College, Peking Union Medical College Hospital, First Affiliated Hospital of Anhui Medical University

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Variable analysis

independent variables

Amplification of the sequence of LukS-PV from PVL-positive Staphylococcus aureus isolates by PCR

dependent variables

Generation of recombinant LukS-PV

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

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