Some Gram-negative pathogenic bacteria such as Salmonella enterica (ATCC9270), Klebsiella pneumoniae (ATCC13883), Escherichia coli (ATCC 2592) and Enterobacter (ATCC 13048) were obtained from German Collection of Microorganisms and Cell Cultures GmbH (DSMZ). The overnight cultures of these bacteria were concentrated and adjusted to 1 × 109 CFU.ml–1 and used separately as prey cells for the examination of the predation potential of Bdellovibrio. Also, the Bdellovibrio suspension in N-[2-Hydroxy Ethyl] Piperazin-Nʹ-2[2-EthaneSulfonic acid] (HEPES) buffer (1 × 105 PFU.ml–) was prepared. HEPES buffer was obtained from Sigma Aldrich and all the medium cultures in this study were obtained from Quelab (12 (link)).
Protocol detail
Predation Potential of Bdellovibrio against Gram-negative Bacteria
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Bacteria
Bdellovibrio
Buffer
Cell cultures
Cells
Cultures medium
Enterobacter
Escherichia coli
Ethanesulfonic acid
Gram negative bacteria
Hepes
Klebsiella pneumoniae
Pathogenic
Piperazin
Salmonella enterica
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Variable analysis
independent variables
- Concentration of Salmonella enterica (ATCC9270), Klebsiella pneumoniae (ATCC13883), Escherichia coli (ATCC 2592) and Enterobacter (ATCC 13048) (all adjusted to 1 × 10^9 CFU/ml)
- Concentration of Bdellovibrio suspension in HEPES buffer (1 × 10^5 PFU/ml)
- Predation potential of Bdellovibrio against the Gram-negative pathogenic bacteria
- HEPES buffer used to prepare Bdellovibrio suspension
- Culture media for the Gram-negative pathogenic bacteria
- Not explicitly mentioned
- Not explicitly mentioned
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