The stable transfected CALU1-FOXF1-AS1 and H1975- FOXF1-AS1 and control cells were prepared for western blot as previously described [25 (link)]. The antibody used included E-cadherin, Vimentin antibody (Cell signaling, USA), GAPDH, FOXF1 and EZH2 antibody produced in rabbit (Abcam).Each of the ratio was determined to the values obtained for GAPDH.
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