Osteosarcoma and Osteoblast Cell Lines

Human osteosarcoma (HOS, 143B, MG-63, Saos-2) [57 (link)–67 (link)] and osteoblast (hFOB1.19) [67 (link)] cell lines were obtained from the American Type Culture Collection (Manassas, VA) and maintained in Dulbecco's Modified Eagle Medium (DMEM, Wako, Osaka, Japan) and DMEM/Ham's F12 (Wako), respectively, both supplemented with 10% fetal bovine serum (FBS). The human osteosarcoma U2OS cell line is from the Human Cancer Cell Line Bank at the Kanazawa University Cancer Research Institute. The cells were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO) containing 10% FBS. This cell line was reported to depend on β-catenin signaling for its proliferation and invasion [31 (link)].

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Shimozaki S., Yamamoto N., Domoto T., Nishida H., Hayashi K., Kimura H., Takeuchi A., Miwa S., Igarashi K., Kato T., Aoki Y., Higuchi T., Hirose M., Hoffman R.M., Minamoto T, & Tsuchiya H. (2016). Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of β-catenin. Oncotarget, 7(47), 77038-77051.

Publication 2016

DMEM DMEM/Ham's F12 RPMI-1640

Cancer Cell line Cells Eagle Fetal bovine serum Human Osteoblast Osteosarcoma Saos β catenin

Corresponding Organization :

Other organizations : Kanazawa University, University of California, San Diego

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Variable analysis

independent variables

Cell lines used: human osteosarcoma (HOS, 143B, MG-63, Saos-2, U2OS) and osteoblast (hFOB1.19)

dependent variables

Proliferation and invasion of human osteosarcoma cell lines

control variables

Cell culture conditions: Dulbecco's Modified Eagle Medium (DMEM) or DMEM/Ham's F12, both supplemented with 10% fetal bovine serum (FBS)
Maintenance of cell lines

controls

Positive control: None specified
Negative control: None specified

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