In vitro PI4KIIIα lipid kinase assays were performed as described earlier (22). Post kinase assay the chloroform-extracted PI(4)P product was separated by thin-layer chromatography (TLC) in n-propanol-2M acetic acid (65:35 v/v). PtdIns was visualized with I2 vapor following PI(4)P detection through autoradiography. PI4KIIIα activity was set as one-fold in control PC3 cells (PC3 scr and PC3-RFP) and compared with CXCR4 manipulated cells.
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