The experimental protocol was approved by the Hebrew University Institutional Animal Care. Induction of DNA damage in vivo, was performed as previously described54 –56 (link). Briefly, for the induction of DNA damage in vivo, C57BL/6 male mice (Harlan, Israel) were injected intraperitoneally (i.p.) with DEN (25 mg/Kg body wt; Sigma-Aldrich). Mice were sacrificed after 48 h, and liver tissues were fixed with 4% PFA.
To study the expression of CD47 in response to DNA damage in tumor tissues, we used a syngeneic orthotropic mouse malignant mesothelioma (MM) disease model63 (link). 1×105 AB12 cells were injected to the peritoneum of BALB/c mice to generate tumors (>95% inoculation success rates). The resultant tumors are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients, such as cisplatin. For cisplatin treatment a single injection of cisplatin (5 mg/kg) was given on day 3 following tumor inoculations, and tumor tissues were harvested from the peritoneum 6 days later.
To study the expression of CD47 in response to DNA damage in tumor tissues, we used a syngeneic orthotropic mouse malignant mesothelioma (MM) disease model63 (link). 1×105 AB12 cells were injected to the peritoneum of BALB/c mice to generate tumors (>95% inoculation success rates). The resultant tumors are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients, such as cisplatin. For cisplatin treatment a single injection of cisplatin (5 mg/kg) was given on day 3 following tumor inoculations, and tumor tissues were harvested from the peritoneum 6 days later.
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