Phylogenetic Diversity of Ca. Hepatincolaceae

To investigate the diversity, host range and environmental distribution of taxa belonging to the ‘Ca. Hepatincolaceae’ family, we downloaded all 47 16S rRNA gene sequences assigned to this family from the SILVA database version SSU r138.1 [76 (link)] (https://www.arb-silva.de/). The sequences were downloaded as ARB alignment and duplicates were removed. The 16S rRNA gene sequences of the three Hepatincola strains were manually added to the alignment, as were seven representatives of the Caedimonadaceae and Paracaedibacteraceae as outgroup. All-gap sites were removed manually. A Maximum Likelihood phylogenetic tree based on the GTR + F + R3 model was produced using IQ-TREE v1.6.1 [73 (link)] with 1000 bootstrap replicates for internal branch support. The tree was visualized in iTOL v6.5.8 [77 (link)].