Evaluating Antitumoral Activity of TK-cAd-MSCs

To evaluate the antitumoral activity, TK-cAd-MSCs were co-cultured with tumoral glioblastoma PG-U87, previously tested in some of our studies [7 (link), 10 (link), 23 (link)]. Both cell types were seeded in co-culture in 96-well plates at a 1:1 cell ratio. Half of the samples received GCV (0.004 μg/μL) (Cymevene; Roche). Every 2 days, the medium was replaced with a fresh medium. Triplicate samples of each co-culture condition were analyzed by BLI on days 1 (before starting GCV treatment), 5, 7, and 9. On day 9, cells were also visualized by fluorescent microscopy. The experiment was repeated three times.

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Villatoro A.J., Alcoholado C., Martín-Astorga M.D., Rubio N., Blanco J., Garrido C.P, & Becerra J. (2022). Suicide gene therapy by canine mesenchymal stem cell transduced with thymidine kinase in a u-87 glioblastoma murine model: Secretory profile and antitumor activity. PLoS ONE, 17(2), e0264001.

Publication 2022

Cymevene

Cells Co culture Glioblastoma Microscopy Tumoral

Corresponding Organization : Andalusian Centre for Nanomedicine and Biotechnology

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Variable analysis

independent variables

GCV (0.004 μg/μL) treatment

dependent variables

Antitumoral activity of TK-cAd-MSCs against glioblastoma PG-U87 cells
Cell viability/proliferation (as measured by BLI on days 1, 5, 7, and 9)
Cell morphology (as visualized by fluorescent microscopy on day 9)

control variables

Co-culture of TK-cAd-MSCs and glioblastoma PG-U87 cells at a 1:1 cell ratio
Cell seeding in 96-well plates
Medium replacement every 2 days

controls

Positive control: Co-culture of TK-cAd-MSCs and glioblastoma PG-U87 cells without GCV treatment
Negative control: Not explicitly mentioned

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