Continuous Cytokine Delivery in EAE

Mice anesthetized with Ketamine and Xylazine were subcutaneously implanted with a mini-osmotic pump (#1007D, Alzet Durect, Cupertino, CA) as previously described^{11 (link)}. This pump system has a reservoir volume of 100 μL and allows for the continuous delivery of the content for 7 days without the need for external connections or frequent handling of animals. Pumps containing 400 ng of rIL-27 or rIL-30 (R&D Systems, Minneapolis, MN) were implanted at day 12 post immunization. Mice with sham surgery were used as controls.

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Kim D., Kim S., Kang M.S., Yin Z, & Min B. (2023). Cell type specific IL-27p28 (IL-30) deletion in mice uncovers an unexpected regulatory function of IL-30 in autoimmune inflammation. Scientific Reports, 13, 1812.

Publication 2023

rIL-27

Animals Delivery Immunization Ketamine Mice Osmotic Surgery Xylazine

Corresponding Organization :

Other organizations : Northwestern University, Zhuhai People's Hospital, Jinan University

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Variable analysis

independent variables

Type of implanted pump (rIL-27 or rIL-30)

dependent variables

Not explicitly mentioned

control variables

Mice anesthetized with Ketamine and Xylazine
Subcutaneous implantation of mini-osmotic pump
Pump system with 100 μL reservoir volume and 7 days of continuous delivery
Pump implantation at day 12 post immunization

controls

Positive control: Mice with sham surgery

Annotations

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