Determination of Plasma Biomarkers

Whole blood was mixed with EDTA-2Na (2 mg/ml) and aprotinin (500 kIU/ml) to determine the plasma levels of FGF15, 5-HT, and insulin. Plasma insulin were measured by enzyme-linked immunosorbent assay (mouse Insulin ELISA Kit [TMB], AKRIN-011T, Shibayagi, Gunma, Japan) as described previously (12 (link)). Plasma levels of FGF15 were measured by enzyme-linked immunosorbent assay (mouse FGF15 ELISA kit, CSB-EL522052MO, WUHAN HUAMEI BIOTECH Co). Plasma 5-HT levels were measured by enzyme-linked immunosorbent assay (mouse 5-HT; BA E-5900, Labor Diagnostika Nord, Nordhorn, Germany). Blood glucose levels were measured using glucose strips (blood glucose monitoring system; Accu-Check, Roche Diagnostics, Tokyo, Japan).

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Nonogaki K, & Kaji T. (2023). Ingestion of whey protein and β-conglycinin exerts opposite effects on intestinal FGF15 and serotonin secretion in mice. Frontiers in Endocrinology, 14, 1080790.

Publication 2023

Accu-Check

Aprotinin Blood Blood glucose Blood system Diagnostics Edta Enzyme linked immunosorbent assay Glucose Insulin Labor Mouse Plasma

Corresponding Organization :

Other organizations : Tohoku University

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Variable analysis

independent variables

Whole blood was mixed with EDTA-2Na (2 mg/ml)
Whole blood was mixed with aprotinin (500 kIU/ml)

dependent variables

Plasma levels of FGF15
Plasma levels of 5-HT
Plasma insulin levels
Blood glucose levels

control variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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