The CGL1 cell line was grown in a humid environment at 37 °C with 5% CO2 [21 (link)]. The CGL1 cells were cultured using 1X Minimal Essential media (Corning, Manassas, VA, USA) with the addition of 50 U penicillin–streptomycin (GIBCO, Grand Island, NY, USA) and 5% calf serum (Hyclone, Logan, UT, USA).
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