ESI-MS was performed on the purified enzymes as described previously (58 (link)). In short, a buffer exchange to 0.1% formic acid (Merck Millipore, Burlington, MA, USA) was performed using centrifugal molecular cutoff filters (Merck Millipore; 10,000 Da). The protein masses were determined using an Orbitrap Fusion Lumos device (Thermo Fisher Scientific). Injection was performed using an EASY-nano LC instrument (Thermo Fisher Scientific) with a 15-cm C18 EASY-spray column. Mass calculations were done using BioPharma Finder 3.0 protein deconvolution software (Thermo Fisher Scientific, Massachusetts, USA).
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